MIRA Summary Report
tiny_test_run_flu_illumina
Barcode Assignment
The ideal result would be a similar number of reads assigned to each test and positive
control. However, it is ok to not have similar read numbers per sample. Samples with a low
proportion of reads may indicate higher Ct of starting material or less performant PCR
during library preparation. What is most important for sequencing assembly is raw count of
reads and their quality.
Automatic Quality Control Decisions
MIRA requires a minimum median coverage of 50x, a minimum coverage of the reference
length of 90%, and less than 10 minor variants >=5%. These are marked in yellow to orange
according to the number of these failure types. Samples that failed to generate any assembly
are marked in red. In addition, premature stop codons are flagged in yellow. CDC does not
submit sequences with premature stop codons, particularly in HA, NA or SARS-CoV-2 Spike.
Outside of those genes, premature stop codons near the end of the gene may be ok for
submission. Hover your mouse over the figure to see individual results.
Median Coverage